FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24117867
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG288497611.961986522274131Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1777150.7368603533637531No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTT633870.2628217495353134TruSeq Adapter, Index 7 (97% over 35bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG482730.2001545161518637Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGCT401770.1665860417921701TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGC369940.15338835727056627TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGAA360030.14927937035227867TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGAC344790.14296040358792922TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGCA326560.13540169203188657TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGCC325580.13499535427407408TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGAT317990.1318483098028528TruSeq Adapter, Index 7 (97% over 35bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCAGAACTCCAG278510.1154787029881208Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGTA266750.11060264989437084TruSeq Adapter, Index 7 (97% over 35bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC258090.10701195093247674Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGA253980.10530782013185494TruSeq Adapter, Index 7 (97% over 35bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTG251200.10415514771683582TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTTGCTA246480.102198092393494TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)