FastQCFastQC Report
Mon 13 May 2024
EGAF00007947800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66329012
Total Bases10 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG11466991.7288045840333035Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAGTGTAATGATACGGCGACCACCGAGATCTACACCGACCATTACACTC1095900.16522181877215358Illumina Single End PCR Primer 1 (96% over 29bp)
AAGAGTGTAATGATACGGCGACCACCGAGATCTACACCGACCATTACACT975240.14703068394867694Illumina Single End PCR Primer 1 (96% over 29bp)
AGTGTAATGATACGGCGACCACCGAGATCTACACCGACCATTACACTAGA883930.1332644605048542Illumina Single End PCR Primer 1 (96% over 29bp)
GTGTAATGATACGGCGACCACCGAGATCTACACCGACCATTACACAGATC830330.12518353205683208Illumina Single End PCR Primer 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)