FastQCFastQC Report
Tue 14 May 2024
EGAF00007947801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66329012
Total Bases10 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG11100941.6736175717497495Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3688710.5561231637220829No Hit
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATGGTCGGTGTAGATC758130.11429840082647395Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATGGTCGGTGT701090.10569884562731012Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)