FastQCFastQC Report
Mon 13 May 2024
EGAF00007947803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74813677
Total Bases11.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG28403123.7965143726326938Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3165940.4231766338660243No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1301720.17399492341487238No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)