FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16868417
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG172296210.214129755032733Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA951530.5640896831042296No Hit
CAAGCAGAAGACGGCATACGAGATTTAGATCGGAAGAGCACACGTCTGAA678610.4022961964954981Illumina PCR Primer Index 11 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTAGATCGGAAGAGCACACGTCTGAAC666500.3951170996069163RNA PCR Primer, Index 29 (96% over 29bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAA449530.2664921077063722TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT424570.25169522427623175Illumina PCR Primer Index 2 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATATAGATCGGAAGAGCACACGTCTGAA420900.24951956072700837Illumina PCR Primer Index 6 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTAAGATCGGAAGAGCACACGTCTGAA361600.21436510610331724RNA PCR Primer, Index 32 (96% over 28bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCT332690.19722656844444858TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAAGATCGGAAGAGCACACGTCTGAAC329250.19518725438196127Illumina PCR Primer Index 10 (96% over 29bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTA303460.1798983271518602TruSeq Adapter, Index 4 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAT269340.15967117720649188TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGCT257180.1524624391251414TruSeq Adapter, Index 4 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTA252410.14963466933500635TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTTA251290.14897070661698725TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAAA241320.1430602527789063TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTT239440.14194574393080275TruSeq Adapter, Index 4 (96% over 33bp)
CAAGCAGAAGACGGCATACGAGATGTAGATCGGAAGAGCACACGTCTGAA232110.13760034507090974RNA PCR Primer, Index 39 (96% over 29bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGA219720.13025525750282319TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATTCAGATCGGAAGAGCACACGTCTGAA219650.13021375983294697RNA PCR Primer, Index 40 (96% over 29bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAAT215960.12802623980661612TruSeq Adapter, Index 4 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTT205180.12163559864568205TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTG204320.1211257701300602TruSeq Adapter, Index 4 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAG202090.11980377293257573TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAAC201540.11947771981211988TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGC200850.11906867135191168TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTTAA196380.11641874871838892TruSeq Adapter, Index 4 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC195360.11581406838590723Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGCATACGAGATAAAGATCGGAAGAGCACACGTCTGAA192910.11436164994024038Illumina PCR Primer Index 2 (96% over 27bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTATA189910.11258317837411773TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTACT189240.11218598639101701TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAC185650.11005774875022357TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTATT182950.10845712434071318TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGCA175510.104046514856729TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGAA175350.10395166303986912TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGT170900.10131359688345384TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTTAT170180.10088676370758443TruSeq Adapter, Index 4 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)