FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16868417
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG16801209.960152158913312Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1534930.9099431203295485No Hit
TAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG1265610.7502837996001641Illumina PCR Primer Index 4 (96% over 26bp)
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA1246730.7390912852106988Illumina Single End PCR Primer 1 (100% over 25bp)
TTATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG681470.4039916727218683Illumina PCR Primer Index 2 (96% over 26bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA653230.3872503270461004No Hit
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA550680.32645624067747436Illumina Single End PCR Primer 1 (100% over 26bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTATGCCTGTGTAGATC435990.2584652727046053Illumina Single End PCR Primer 1 (96% over 33bp)
GAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG411180.24375731285277094Illumina PCR Primer Index 2 (96% over 26bp)
TCATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG405300.24027150858317056Illumina PCR Primer Index 3 (96% over 26bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTATGCCTGTGT341200.2022714994536832Illumina Single End PCR Primer 1 (96% over 33bp)
TGATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG313990.1861407623489507Illumina Paired End PCR Primer 2 (96% over 26bp)
CATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA237470.14077788093571555Illumina Single End PCR Primer 1 (100% over 25bp)
GTATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG228270.13532390146627274Illumina PCR Primer Index 2 (100% over 26bp)
GATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA208820.1237934774792442Illumina Single End Adapter 1 (96% over 25bp)
AAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG203810.12082342996381937Illumina PCR Primer Index 4 (96% over 26bp)
AATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA197860.11729612802434278Illumina Single End PCR Primer 1 (100% over 25bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG187900.11139160242481555Illumina Single End PCR Primer 1 (100% over 32bp)
CTATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG176110.10440220916995352Illumina Paired End PCR Primer 2 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)