FastQCFastQC Report
Sat 18 Mar 2023
EGAF00007947806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86852519
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG28770633.3125844052951416Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCA1958290.22547302283771414TruSeq Adapter, Index 8 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCAAAA1350170.15545547965050963TruSeq Adapter, Index 8 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCAAA1175900.13539043122053834TruSeq Adapter, Index 3 (96% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCAAAT1140920.13136291418329502TruSeq Adapter, Index 8 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCAATA1081120.12447767922540047TruSeq Adapter, Index 8 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCAAAC977670.11256668329907624TruSeq Adapter, Index 8 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCAAT936460.10782185833896193TruSeq Adapter, Index 3 (96% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCAATT931760.10728071110982976TruSeq Adapter, Index 8 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)