FastQCFastQC Report
Sat 25 May 2024
EGAF00007947807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86852519
Total Bases13.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG28157423.2419808111725583Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8802351.0134824068833284No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAAGACGGTGT955910.11006128676590256Illumina Single End PCR Primer 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)