FastQCFastQC Report
Sat 25 May 2024
EGAF00007947808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106593488
Total Bases16 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG99553439.339541454915144Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGT6801010.6380324096346299TruSeq Adapter, Index 13 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTAA4299670.40337079503393297TruSeq Adapter, Index 13 (97% over 36bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTG3258000.3056471892541878TruSeq Adapter, Index 13 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTA3105260.2913179837027192TruSeq Adapter, Index 13 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTAC2980740.27963621942833883TruSeq Adapter, Index 13 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTAT2924620.2743713574697922TruSeq Adapter, Index 13 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTTA2844680.2668718374240648TruSeq Adapter, Index 13 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTA2729740.2560888147313464TruSeq Adapter, Index 4 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTAAA2501190.23464754244649544TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTAAC2408180.22592186869802028TruSeq Adapter, Index 13 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTC2352280.22067764589896896TruSeq Adapter, Index 4 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTAAT2275880.21351022869239442TruSeq Adapter, Index 13 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTT2226900.2089152012738339TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTACT2182260.20472732818350028TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTTAA1992310.1869072902464736TruSeq Adapter, Index 13 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTCT1983040.1860376311168277TruSeq Adapter, Index 13 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTT1965750.18441558080921416TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTATA1940680.18206365477035522TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTACA1842300.1728341979014703TruSeq Adapter, Index 13 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTTT1818430.17059484909622247TruSeq Adapter, Index 13 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTCA1768400.16590131659825225TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTATT1722320.16157835082758526TruSeq Adapter, Index 13 (97% over 36bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1720120.16137195923263153Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTT1697930.15929021855443928TruSeq Adapter, Index 4 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTTAT1668070.15648892172474926TruSeq Adapter, Index 13 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTAG1662020.15592134483862655TruSeq Adapter, Index 13 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTGA1640070.15386211960715648TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTATC1625820.15252526495802446TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTTAC1603270.15040975110974883TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTACC1572500.14752308321123705TruSeq Adapter, Index 13 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTTC1514080.14204244822160242TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTCTT1482830.13911074942964619TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTCTA1468880.13780203908891694TruSeq Adapter, Index 13 (97% over 36bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGC1423350.13353067121698842TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTTTA1408130.13210281663735404TruSeq Adapter, Index 13 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTGC1398750.1312228379279605TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTTCT1385990.1300257666772289TruSeq Adapter, Index 13 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTG1350910.12673475888133054TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTCAA1317650.1236144932230757TruSeq Adapter, Index 13 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTGT1297000.12167722666135102TruSeq Adapter, Index 13 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTTG1280540.12013304227365183TruSeq Adapter, Index 13 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTG1206100.11314950121530877TruSeq Adapter, Index 4 (96% over 33bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTC1203880.11294123333312818TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTGAA1190060.11164471885937348TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTAAG1181350.11082759577207943TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTTTT1149380.10782835063995655TruSeq Adapter, Index 13 (97% over 36bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTT1148690.10776361873063015TruSeq Adapter, Index 13 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTTCA1090230.1022792311665418TruSeq Adapter, Index 13 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT1079820.10130262366496534Illumina PCR Primer Index 2 (96% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTCAT1076040.10094800537909032TruSeq Adapter, Index 13 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)