FastQCFastQC Report
Wed 22 May 2024
EGAF00007947809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106593488
Total Bases16 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG93125808.736537451518615Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11530621.0817377511842No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGAGACGAGTGT2474050.23210142067965728Illumina Single End PCR Primer 1 (97% over 36bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGAGA1805700.16940059227633117Illumina Single End PCR Primer 1 (97% over 36bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGAGACGA1778540.16685259422226617Illumina Single End PCR Primer 1 (97% over 36bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1588100.14898658724818162Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGAGACGAGTG1335030.12524498682321006Illumina Single End PCR Primer 1 (97% over 36bp)
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA1239920.11632230291591546Illumina Single End PCR Primer 1 (100% over 25bp)
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA1215840.11406325309478568Illumina Single End PCR Primer 1 (100% over 26bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGAGACGAGTGTAGA1137860.1067476091972898Illumina Single End PCR Primer 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)