FastQCFastQC Report
Fri 10 May 2024
EGAF00007947812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15392683
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG216161014.043100868120263Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3232892.1002771251769428No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA424300.2756504502821243No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA379870.24678608661011212No Hit
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC300120.19497575568859568No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACA295200.19177943182484822TruSeq Adapter, Index 13 (97% over 35bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT275180.17877325220041237Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCA255180.1657800657624145TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTCT247140.1605568048143394TruSeq Adapter, Index 13 (97% over 37bp)
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG225920.14677103400362368No Hit
ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC218540.14197654820800246No Hit
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC214250.13918950971705193No Hit
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC213620.138780224344255Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTAC212240.13788369448003313TruSeq Adapter, Index 13 (97% over 37bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA211230.13722753856491424No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTAA204520.13286832451496597TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTCA202850.13178339344739318TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTTA202560.1315949922440422TruSeq Adapter, Index 13 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACT198120.12871050485480665TruSeq Adapter, Index 13 (97% over 35bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT192490.12505292287251027Illumina PCR Primer Index 2 (96% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACACT190000.12343527116097953TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTTT187200.12161622505965984TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTAT184660.11996609038203412TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACATC183580.11926445831438223TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACAAA179810.11681524267081964TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACCTC176430.114619394162798TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACATA175760.11418412241712508TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACGCT173340.11261194685812732TruSeq Adapter, Index 13 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTA168970.1097729356214248TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTGC168110.10921422860459089TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTTC167320.10870099774028999TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACAAC166510.10817477368955106TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACAAT166290.10803184863873308TruSeq Adapter, Index 13 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACAA164160.10664807428308633TruSeq Adapter, Index 13 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCAC158100.10271113879237297TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACACA157080.1020484862840351TruSeq Adapter, Index 13 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)