FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18594828
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG319326717.17287731835971Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2038681.0963693775494994No Hit
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACT484980.2608144587301372TruSeq Adapter, Index 11 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAG475260.2555871987630109TruSeq Adapter, Index 11 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC368600.19822716295090226Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG257960.13872674702879748Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGCA234220.12595975612143334TruSeq Adapter, Index 11 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGC230030.12370644138251777TruSeq Adapter, Index 11 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGCT220000.1183124683917485TruSeq Adapter, Index 11 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGAC216020.11617208828175232TruSeq Adapter, Index 11 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGCC212190.11411237576384142TruSeq Adapter, Index 11 (97% over 37bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTT209020.11240760065110579TruSeq Adapter, Index 11 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGG208340.11204190756698583TruSeq Adapter, Index 11 (97% over 37bp)
GAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA207680.11168697016181058TruSeq Adapter, Index 7 (97% over 35bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT200550.10785257061802346Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC200100.10761056784176762Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG191910.10320611731391117Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGTA189440.10187779096424016TruSeq Adapter, Index 11 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)