FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18594828
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG314671916.922549646600658Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1374600.739237813869534No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG873000.4694853859363475No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG351420.18898803473740117Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTCT347030.18662716320903855Illumina Single End PCR Primer 1 (96% over 33bp)
CCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACAC326110.17537672303287774No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT245850.13221418342777894Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTCTTCGGTGT244830.13166564380159904Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTCTTCG217410.11691960796840928Illumina Single End PCR Primer 1 (96% over 33bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214060.11511803174517128No Hit
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG199780.10743847697865234No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT198170.10657264482360362Illumina Single End PCR Primer 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)