FastQCFastQC Report
Sat 18 Mar 2023
EGAF00007947818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116522226
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1818876715.609697500972905Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCT8667780.7438735336209592TruSeq Adapter, Index 16 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAAA6773350.5812925338381366TruSeq Adapter, Index 16 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAA5208060.4469585055815875TruSeq Adapter, Index 16 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTA4357140.3739320942941821TruSeq Adapter, Index 16 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAAC4242910.36412881436027494TruSeq Adapter, Index 16 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAAT4144990.35572526738375215TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAACT3896280.33438084164303555TruSeq Adapter, Index 16 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATA3855610.3308905204059524TruSeq Adapter, Index 16 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAA3833180.3289655657625353TruSeq Adapter, Index 6 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAAAT3275400.28109658667179943TruSeq Adapter, Index 16 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTACA3267690.2804349103320426TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAACA3176540.27261236839055925TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAAAC3130940.26869895190639426TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTACAA3079030.26424400783417923TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTACAT3075980.2639822551965322TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAAAA3032570.26025678568825145TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTACTT2983440.2560404227087114TruSeq Adapter, Index 16 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAT2972810.25512815040110887TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTACTA2936560.25201715593727153TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAATT2900090.2488872809553089TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAACC2825470.2424833524893354TruSeq Adapter, Index 16 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTACT2670120.229151132076725TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATCT2653390.22771535449382851TruSeq Adapter, Index 16 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAC2626070.22537073742480684TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAATA2612520.2242078691493587TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTACCT2397200.2057289911368497TruSeq Adapter, Index 16 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAT2375180.20383922291357529TruSeq Adapter, Index 6 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAG2367240.20315780785032378TruSeq Adapter, Index 6 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATAT2357130.20229016222192667TruSeq Adapter, Index 16 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAAG2350310.20170486616004055TruSeq Adapter, Index 16 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATT2314550.19863592375930064TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATAA2288780.19642432852252584TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAGCT2244100.1925898669323396TruSeq Adapter, Index 16 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGT2235500.19185181031471196TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTACCA2209320.18960502865779444TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATTT2183660.1874028736800823TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATTA2167620.18602631226766986TruSeq Adapter, Index 16 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAGA2158760.18526594231043955TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTACTC2132590.18302001885889135TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAATC2082850.17875130535182188TruSeq Adapter, Index 16 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATC2076070.17816944211141314TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATCA2075660.17813425569127042TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTACAC2030910.17429378666349885TruSeq Adapter, Index 16 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1964770.16861761635072092Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAC1948580.1672281818577685TruSeq Adapter, Index 6 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTACC1873320.16076932824815757TruSeq Adapter, Index 16 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATG1855100.15920567806523023TruSeq Adapter, Index 16 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAG1829020.15696747846200604TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAATG1827820.1568644938176859TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTACCC1825910.15670057659214304TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAGCA1806580.15504166561321955TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAAGT1793930.15395603582101153TruSeq Adapter, Index 16 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAGT1761290.15115485349550395TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATGA1715860.14725602650261763TruSeq Adapter, Index 16 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAGC1702400.14610088207549346TruSeq Adapter, Index 16 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT1688930.144944879443Illumina PCR Primer Index 2 (96% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTACTG1665620.14294440272708145TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAAGA1657430.14224153252959656TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAAAG1633400.14017926502708591TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATGT1607140.1379256177272137TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATGC1603820.13764069354459466TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATTC1598520.13718584469884743TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAGTA1586350.1361414087643674TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAGAT1583370.13588566356430576TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTACAG1571820.13489443636272447TruSeq Adapter, Index 16 (97% over 37bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTC1561260.13398817149270734TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAGTT1554680.13342347235968527TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAGAA1551880.13318317485627162TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAGCC1520140.13045923101400414TruSeq Adapter, Index 16 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT1507120.12934184762313072Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAAGC1449930.1244337711159071TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAGAC1376020.11809077523115633TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATAC1358710.11660522173683842TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATAG1353240.1161357833998125TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTACGT1346590.11556507682920508TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAGTC1326600.11384952429590557TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATTG1322220.11347363034413709TruSeq Adapter, Index 16 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG1260080.10814074217909293Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1237340.10618918316922644Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTATCC1220460.10474053250578992TruSeq Adapter, Index 16 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTCTAAGG1218560.10457747348561637TruSeq Adapter, Index 16 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)