FastQCFastQC Report
Sat 18 Mar 2023
EGAF00007947819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116522226
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1671345314.343575104718647Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13881481.1913160670308514No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAAGAAGCGTGT6913010.5932782300262612Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAAGAAGCGTG2367320.20316467349327846Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAAGAAGC1815320.15579173710601787Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1785370.15322141202486125Illumina Single End PCR Primer 1 (100% over 32bp)
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA1780340.15278973472408602Illumina Single End PCR Primer 1 (100% over 26bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAAGAAGCGTGTAGATC1648650.14148802821532092Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT1628480.13975702798537337Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT1399580.12011270708130825Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT1300280.11159072776381734Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAAGAAGCGT1175300.10086487705787564Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)