FastQCFastQC Report
Fri 3 May 2024
EGAF00007947820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18249796
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG14919388.175094121600045Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1377460.7547810397442252No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGA549620.3011650102828547TruSeq Adapter, Index 25 (97% over 35bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGT432400.23693415531877726TruSeq Adapter, Index 25 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAA354870.19445148866321574TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCTA302590.16580459310339687TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCAA279260.15302088856226118TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGACT277580.1521003303269801TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCAT261650.14337146563172543TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAAA258520.14165637796718386TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCTC258090.14142075889505834TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCTT250250.13712482046374655TruSeq Adapter, Index 25 (97% over 40bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTA247680.1357165855442987TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAAT237520.13014940002617015TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAAC234780.12864801338053314TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCAC232820.12757402877270518TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGACA232640.12747539753321077TruSeq Adapter, Index 25 (97% over 40bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGC209910.11502046379038976TruSeq Adapter, Index 25 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGTCT208570.11428620900748698TruSeq Adapter, Index 25 (97% over 40bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGG203620.1115738499213909TruSeq Adapter, Index 25 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCCA202210.11080123854535141TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGATA201820.11058753752644687TruSeq Adapter, Index 25 (97% over 40bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCA193930.10626420152860887TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGCCT192430.10544227453282218TruSeq Adapter, Index 25 (97% over 40bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGTAA190900.10460390899711974TruSeq Adapter, Index 25 (97% over 40bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGTA189150.10364499416870195TruSeq Adapter, Index 25 (97% over 40bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGCTAGAT186000.10191894747754988TruSeq Adapter, Index 25 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)