FastQCFastQC Report
Fri 10 May 2024
EGAF00007947822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13884018
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG10169447.324565554438204Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4067582.9296850522665703No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTGGA326850.2354145608281407TruSeq Adapter, Index 16 (97% over 37bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG163530.1177829069365943No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155610.11207850638050167No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)