FastQCFastQC Report
Fri 3 May 2024
EGAF00007947828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17189187
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4954202.8821607444261326No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG3325921.9348908124625093Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261550.15215961057378688No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247390.14392187367558454No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246230.14324703082234197No Hit
CTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225630.13126275256648265No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)