FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12303033
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG165012013.41230247858394Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGT837060.6803688163723531TruSeq Adapter, Index 3 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGA784240.6374363134683944TruSeq Adapter, Index 3 (96% over 33bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA464190.37729720793238547No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTC450770.3663893285501226TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGC427630.34758095828890323TruSeq Adapter, Index 3 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAA424760.34524820017958174TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTG413510.3361041135141229TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTA370410.3010721014891206TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGG346630.28174353429759963TruSeq Adapter, Index 3 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTGC326040.265007823680551TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTGT319780.25991964745603785TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCA298570.24267999606275947TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTT297520.2418265479739833TruSeq Adapter, Index 1 (97% over 36bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA287850.2339666974802067No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCT270220.21963689766580324TruSeq Adapter, Index 1 (97% over 36bp)
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC258000.20970438752785595No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA257310.20914355021237446No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGT242680.19725217350875998TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCTC238820.1941147357728781TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGT235340.19128616496436285TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTTA232970.18935981070683952TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGA232210.1887420768521063TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAT230220.18712458952194957TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTTT227830.18518197911035433TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGCT226560.18414971332678698TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAG226450.1840603044793914TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTAT222080.18050833481467538TruSeq Adapter, Index 1 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCG222000.18044331019838766TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG220200.17898025633191425Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTAC220100.17889897556155462TruSeq Adapter, Index 1 (97% over 36bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA217640.17689946861070763No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTAA214470.17432286819030723TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGA207810.16890956888435557TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGC205910.16736523424752253TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAC205280.16685316539425685TruSeq Adapter, Index 1 (97% over 36bp)
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC203690.16556080114553867No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCAC201370.16367508727319516TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAAA199410.16208198417414632TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCAT196570.15977361029593273TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTGA195970.15928592567377492TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCG195020.1585137583553584TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAAT194980.15848124604721453TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGTG191670.15579085254831065TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCAA191440.1556039067764835TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCTA190260.15464479368623982TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTCT187750.152604646350213TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTGG183930.149499720922475TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTC182170.1480691793641454TruSeq Adapter, Index 1 (97% over 36bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCT180310.14655735703545622TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT179200.1456551404844643Illumina PCR Primer Index 2 (96% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCTT176340.14333051045217873TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTTG174940.14219257966714385TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGACT172600.14029060964072843TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGC167660.1362753395849625TruSeq Adapter, Index 1 (97% over 36bp)
ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC164240.13349553723866303No Hit
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGG163830.1331622860801885TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGCA163560.1329428280002175TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTCA160910.13078888758568719TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTAG160280.1302768187324215TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGTT160120.13014676949984608TruSeq Adapter, Index 1 (97% over 36bp)
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG159610.12973223757101196No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCG159470.12961844449250848TruSeq Adapter, Index 1 (97% over 36bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC158820.12909011948517085Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG157430.12796031677717193Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGTA156120.12689553868546075TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAAC155190.12613962752111613TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGACA153620.12486351942646988TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCC153430.12470908596278658TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGAA152940.12431081018802438TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGTTC150150.12204307669499057TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGG149260.12131967783878983TruSeq Adapter, Index 1 (97% over 36bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT147690.12004356974414357Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGGC145310.11810908740958428TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAGT140440.1141507138930701TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGAT138650.11269578810363266TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCGT136250.11074504961500144TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGCC135590.11020859653062785TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAAG135490.11012731576026823TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCCA133520.10852608458418343TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCCT133340.10837977919753608TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCCC133150.10822534573385277TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGCGC132960.10807091227016948TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGATA131680.10703051840956616TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGAC131430.10682731648366708TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATTAGATCGGAAGAGCACACGTCTGAAC130180.10581130685417164RNA PCR Primer, Index 29 (96% over 29bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGAGC129770.10547805569569715TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGATG128800.1046896322232087TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTCGGGT123330.10024357408453671TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)