FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12303033
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG162725913.22648650946478Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3486092.833520807430168No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA395640.3215792398508563No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCGTGT378480.30763145965714306Illumina Single End PCR Primer 1 (96% over 33bp)
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC324290.26358541019925735No Hit
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC300260.2440536410818373No Hit
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA281590.22887852125569363Illumina Single End PCR Primer 1 (100% over 25bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA266240.2164019230054898No Hit
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA249940.2031531574368694Illumina Single End PCR Primer 1 (100% over 26bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCGTGTAGATC237670.19318000691374232Illumina Single End PCR Primer 1 (96% over 33bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC223270.18147557598195502No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT222970.18123173367087614Illumina Single End PCR Primer 1 (96% over 26bp)
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA220970.17960611826368345No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA215990.17555833589977365No Hit
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCAATCGTG166930.13568198996133718Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG158210.12859430678597708Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT151910.12347361825332014Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT151880.12344923402221224No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAG147160.11961278166123751No Hit
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG140820.11445958082043671No Hit
TGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG136020.11055810384317429No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGGTCGTGTAGGGAAAGAGT126290.1026494848871819No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)