FastQCFastQC Report
Sun 5 May 2024
EGAF00007947836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17838044
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG13104867.346579030750232Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA808720.45336809349724666No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGAGT195770.10974858005732019TruSeq Adapter, Index 3 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)