FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22592320
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG367558216.26916580501693Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGT1200030.5311672285095113TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAA609730.26988374810555094TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTA591720.2619120125777255TruSeq Adapter, Index 6 (97% over 36bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG569530.25209009079191513TruSeq Adapter, Index 6 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA553240.2448796759252702No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTA499100.2209157802297418TruSeq Adapter, Index 3 (96% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAT498330.22057495644537614TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTT481360.21306355434059007TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTGC471310.20861514001218112TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAC451550.19986880497443377TruSeq Adapter, Index 6 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTT425460.1883206328522259TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTA419460.18566486310392205TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTC398340.1763165535898925TruSeq Adapter, Index 3 (96% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTCT393300.17408570700131726TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTT391110.17311635104318637TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTT356920.15798288976076824TruSeq Adapter, Index 3 (96% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTC356470.15778370702964548TruSeq Adapter, Index 6 (97% over 36bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC355980.15756681916686732Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTGA344660.1525562669084007TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTGT340430.15068394923584652TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAG338490.14982525035056163TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTG335660.14857261228594495TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTT312780.13844527697907963TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTAA305810.13536015778813332TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTG294400.13030976898344215TruSeq Adapter, Index 3 (96% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAAC294130.1301902593447685TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTG291560.12905270463591167TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAAT288650.1277646563079843TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTAAA286780.12693694140309628TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTAC281410.12456002747836432TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTGCT280670.12423248254274019TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTACT278850.12342689905242137TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTAT269820.11942996558122407TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG268080.11865979235421593Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTCA262930.11638025665358849TruSeq Adapter, Index 6 (97% over 36bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC262350.11612353224458576Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTTA249520.11044461126612937TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTGCA239100.10583242446990836TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTCT239060.105814719338253TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTTTT234120.10362813557881617TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGAGTTCTC230420.10199041090069545TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)