FastQCFastQC Report
Wed 1 May 2024
EGAF00007947839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22592320
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG341340915.108713934646817Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3137461.3887285590855654No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT702390.31089768558519Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGACCGTGTGT413280.18292942026316908Illumina Single End PCR Primer 1 (97% over 34bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGACCGTGTGTAGATC399990.17704689027067605Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT396370.17544457585586606No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350730.15524302063710144No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG323420.1431548419994051Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGAC312950.1385205237886149Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGACCGT280400.12411297290406652Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT277840.12297984447812353Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGACCGTGTG270580.11976636308267588Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)