FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24746842
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG862407134.84917792743009Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTAT2893141.1690946262961552TruSeq Adapter, Index 6 (97% over 36bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG1433770.5793749360019351TruSeq Adapter, Index 6 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATG1327770.5365411877604424TruSeq Adapter, Index 6 (97% over 36bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1167410.4717410003264255Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCA992740.40115825687980716TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGC983800.39754567471679825TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCAA839020.3390412400903517TruSeq Adapter, Index 6 (97% over 36bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT805460.3254799137603093Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGC757600.3061400723373108TruSeq Adapter, Index 3 (96% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCT745110.30109296370017635TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCC744990.3010444726644313TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGAC736800.29773495947482914TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGAA733850.29654288817942914TruSeq Adapter, Index 6 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGA707740.28599204698522745TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCAC689830.2787547599002733TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCAT684440.2765767042113899TruSeq Adapter, Index 6 (97% over 36bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC654390.2644337406768912Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGT651310.26318913742610067TruSeq Adapter, Index 6 (97% over 36bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT646370.2611929231212613Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCCA640770.2589300081198239TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCTA640210.25870371661968017TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGACA636360.25714796255619204TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTA611450.2470820317194412TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGAT608370.24583742846865067TruSeq Adapter, Index 6 (97% over 36bp)
GAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC606660.2451464312092832TruSeq Adapter, Index 5 (97% over 35bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGA600340.2425925699933753TruSeq Adapter, Index 3 (96% over 32bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGT599030.2420632095198248TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGAAC591310.2389436195535576TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTC581090.2348137996759344TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGACC571780.2310517034860448TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG548780.22175758830156994Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGACT528950.21374444464469447TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGATC525310.21227354989376018TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGAAA522330.21106935583942388TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTCA518260.20942470154373638TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCCC516220.20860035393606993TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTAC514470.20789319299812076TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTT506730.20476552119256267TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTAA496810.2007569289043022TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCTT495900.20038920521656864TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCTC484730.19587549797263018TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCG477580.19298624042615214TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGAAT469900.18988281413846664TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGT465920.18827452811958795TruSeq Adapter, Index 3 (96% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCCT458650.1853367795373648TruSeq Adapter, Index 6 (97% over 36bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTA437300.1767094160943849TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTTC423590.1711693152605088TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTCT421960.1705106453583047TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGATA419220.16940343337545857TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTAT416720.1683932034641026TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG411470.16627172065025508Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT408100.16490993072974727Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTTA402600.16268742492476412TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCAG392210.15848890941316876TruSeq Adapter, Index 6 (97% over 36bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCC391050.15802016273429959TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTCC388420.1569574008675531TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGATT374170.15119909037282414TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCGA366820.1482290144334376TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGGC358140.1447214961812097TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT357090.1442971996184402Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTTT344790.13932686845456885TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCGC340700.1376741323195905TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATG320160.12937408336788994TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGGCTGAACTCCAG311000.12567260097268168Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGTG309200.12494523543650539TruSeq Adapter, Index 6 (97% over 36bp)
CAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT309170.12493311267756912Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCGT307940.12443607956118198TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTACAG305700.12353091356060704Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGAG304590.12308237147996501TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCTG289440.11696037821714786TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGGCC287790.11629362647565293TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGGCA279650.11300431788427792TruSeq Adapter, Index 6 (97% over 36bp)
CAAGAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT279140.1127982309823613Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGG277150.11199408797292196TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGG269940.10908058490857137TruSeq Adapter, Index 3 (96% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGAGC269360.10884621156913678TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGCCG268590.10853506075643915TruSeq Adapter, Index 6 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGAAG253500.10243731301149456TruSeq Adapter, Index 6 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTATGGA253400.10239690381504032TruSeq Adapter, Index 6 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATAGGAAGAGCACACGTCTGAACTCCAG253000.10223526702922336Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGAGATCGGAAGAGCACACGTCTGAACTCC251700.10170994747531825Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)