FastQCFastQC Report
Wed 1 May 2024
EGAF00007947841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24746842
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC66

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG787138931.807650446873183Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5110012.064913979731232No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGT2039090.8239798839787316Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGT1320150.5334620069906293Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT1049210.42397733011751554Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTG1017220.41105042817180476Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1010100.40817329338426295Illumina Single End PCR Primer 1 (100% over 32bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGTAGATC852340.3444237450580563Illumina Single End PCR Primer 1 (96% over 33bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGTAGA738080.2982521971894434Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGAT737520.29802590568929965Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT707870.28604457894061797Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT622310.2514704704543715No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG585350.23653523144488495Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGT519840.21006316684771334Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG480960.19435207126630544Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT462320.18681979704723536Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT458040.18509028343899395Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTG413980.16728599148125647Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGAGTAGGGAAAGAG412250.16658691238259815Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTG399790.16155192650440003Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGACAGAG399580.16146706719184614Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGGTCGTGTAGGGAAAGAGT368730.14900083008571355No Hit
CGTATGCCGTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGG351220.1419251797865764Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATG342650.13846211165044817Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCTGTAGGGAAAGAGT327910.1325057960930934No Hit
CGTATCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT301470.12182160455059277Illumina Single End PCR Primer 1 (100% over 31bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGTAGATC300360.12137306246995071Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT285710.11545311518940478Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT267570.10812288695260591Illumina Single End PCR Primer 1 (100% over 31bp)
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTGTGTA263220.10636508690684654Illumina Single End PCR Primer 1 (97% over 35bp)
TCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGATGGTG248690.10049363066204568Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)