FastQCFastQC Report
Wed 1 May 2024
EGAF00007947842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24273199
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG280691011.563823952500039Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCG1120440.461595523523702TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCT735090.3028401818812592TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCA728710.3002117685435694TruSeq Adapter, Index 7 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA704190.29011009220498707No Hit
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACA607380.2502265976561227TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTA588640.24250614844792398TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCC578080.23815567119933387TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTG561320.2312509364752458TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCTC558640.23014683808261122TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAA538560.2218743396780952TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGC533320.2197155801342872TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTAC529980.2183395769136157TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCAC520790.21455350817170823TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCTA512230.21102698494747232TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAC508460.2094738316115647TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTGC495560.20415932815448018TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCAA484350.19954106584797493TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGC481850.19851112331753223TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTT480740.19805382883401565TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAT463570.19098018353493496TruSeq Adapter, Index 7 (97% over 35bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGG458680.188965615945389TruSeq Adapter, Index 7 (97% over 35bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGA453780.18694692858572123TruSeq Adapter, Index 10 (96% over 33bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC447060.18417844306389117Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCAT446120.1837911846724447TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTC430330.1772860676501684TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCCC420910.1734052441954602TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAAC418940.1725936494814713TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGT408240.16818549545117642TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCTT398270.1640780846397708TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCCA390800.1610006163588079TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGACT387080.15946806187350915TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTGT370220.15252212944820334TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGCT368420.15178057082628457TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCCT353510.14563799357472412TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTCT346910.1429189452943553TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTTA341850.14083434161273922TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGACC333210.13727486022752913TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGACA327670.13499250758006806TruSeq Adapter, Index 7 (97% over 35bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGT321630.1325041664265184TruSeq Adapter, Index 10 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTAT318280.13112404343572512TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCG318030.13102104918268087TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAGC314450.12954617147908687TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGCC312650.1288046128571681TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAG312310.12866454067302788TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAAA311730.12842559400596518TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTTT309090.12733797469381766TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTGA305910.1260278877950945TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTAA304290.12536048503536762TruSeq Adapter, Index 7 (97% over 35bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT304070.12526985009268865Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGT295280.121648572155652TruSeq Adapter, Index 7 (97% over 35bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG293630.12096881008555979Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTCC293400.12087405537275907TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGATG287420.11841043283994006TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGTTC286570.11806025237958952TruSeq Adapter, Index 7 (97% over 35bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAT282160.11624343375588855TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGAAT281600.11601272662906936TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGA280680.11563370777786645TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGCA277550.11434421972975213TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGATA273690.11275398846274858TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGATC268570.11064466616040183TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGCGC260840.10746008385627293TruSeq Adapter, Index 7 (97% over 35bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT259310.10682975902764197Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACATCGGGA248040.10218677810040612TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)