FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24273199
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG273898411.283984447208626Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2818521.1611654483613798No Hit
CCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAG1169520.4818153552813538Illumina Single End PCR Primer 1 (96% over 33bp)
CGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAG1000910.41235191125817405Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGT747300.30787042119994157Illumina Single End PCR Primer 1 (96% over 33bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAGATC569260.2345220339519319Illumina Single End PCR Primer 1 (96% over 33bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA533220.2196743824330695No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG433610.17863735225010927Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCAC379310.1562670004888931Illumina Single End PCR Primer 1 (96% over 33bp)
CGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAG366220.15087422139949497Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTG347850.1433062036858018Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT317860.13095101309061077Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTT317010.13060083263026023Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT304060.12526573032256688Illumina Single End PCR Primer 1 (100% over 31bp)
TGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCACGTTGTGTAG272320.11218957995606596Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)