FastQCFastQC Report
Wed 1 May 2024
EGAF00007947845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27350499
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG438794516.04338187760304Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5247361.9185609739697986No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTACAGCGTGT957720.3501654576759276Illumina Single End Sequencing Primer (100% over 33bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTACAGCGTGTAGATC682600.24957497119156763Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT582630.21302353569490634Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTACAGC477620.17462935502566151Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG444040.16235169968928173Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTAC432180.15801539854903562Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTACAGCGTG374960.13709439085553796Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT331390.12116415133778728Illumina Single End PCR Primer 1 (100% over 31bp)
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA300050.10970549385588907Illumina Single End PCR Primer 1 (100% over 25bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT281230.10282444938207524Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT279740.10227966955922815No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)