FastQCFastQC Report
Sat 25 May 2024
EGAF00007947847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79218272
Total Bases11.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG4427650.558917770890029Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3822400.4825149430171868No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG909570.11481820759735835No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)