FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17583332
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG5376413.057674165510837Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1377170.7832247039412097No Hit
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA778100.44252136057034014Illumina Single End PCR Primer 1 (100% over 26bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA758290.43125500900511915No Hit
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA427090.24289480514842124Illumina Single End PCR Primer 1 (100% over 25bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGTAACTGTGT390140.22188058554544723Illumina Single End PCR Primer 1 (96% over 33bp)
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA187550.10666351519723338Illumina Single End PCR Primer 1 (100% over 26bp)
TAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG185280.10537251983867449Illumina PCR Primer Index 4 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)