FastQCFastQC Report
Sat 18 Mar 2023
EGAF00007947852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89217924
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG34428573.8589297370335585Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCCT1848330.20717025426415436TruSeq Adapter, Index 14 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCCTAA1780500.19956752188046875TruSeq Adapter, Index 13 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCCTAT1472650.16506212361542957TruSeq Adapter, Index 13 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCCTAG1132410.1269262889371871TruSeq Adapter, Index 13 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCCTAC1071790.12013169012988914TruSeq Adapter, Index 13 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)