FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23761800
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG492757420.73737679805402Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT1397360.5880699273624052Illumina PCR Primer Index 2 (96% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAAA1224500.5153229132473129TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAA1171600.4930602900453669TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAAT1053000.44314824634497385TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTAA958330.40330698852780517TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATA893780.37614153809896556TruSeq Adapter, Index 18 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGAT870270.3662475065020327TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAAC849760.3576160055214672TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTCT835560.3516400272706613TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCACA803960.3383413714449242TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCACT787690.33149424706882474TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTTA783740.3298319150906076TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATT756440.3183428864816638TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCAA727830.30630255283690627TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTAC707600.29778888804720177TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTCA706930.29750692287621305TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTA704050.29629489348450033TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCA703980.2962654344367851TruSeq Adapter, Index 18 (97% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCAT658000.27691504852326004TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATC639310.2690494827832908TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAT631270.2656659007314261TruSeq Adapter, Index 18 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA607120.25550252926966815No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCTA583670.24563374828506257TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTAT579530.24389145603447548TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCAC560640.23594172158674848TruSeq Adapter, Index 18 (97% over 38bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT557140.23446876920098647Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTTT556650.23426255586697975TruSeq Adapter, Index 18 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATC542040.22811403176527031TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCCT540450.22744489053859557TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCCA532440.22407393379289448TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGAA504980.21251757021774448TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCTT503610.21194101456960332TruSeq Adapter, Index 18 (97% over 38bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC494260.2080061274819248Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCACC492470.2072528175474922TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAC490830.20656263414387796TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGAT479500.20179447684939691TruSeq Adapter, Index 18 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCA477270.20085599575789714TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTGA471790.19854977316533257TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAAG464910.19565436961846322TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCTC459640.19343652416904444TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCA456620.1921655766819012TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTTC445940.18767096768763308TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAGA438250.18443468087434453TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGAGATCGGAAGAGCACACGTCTGAACTCC429850.18089959514851567Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATG416290.17519295676253482TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAGT413520.17402722016008887TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTAG412730.17369475376444546TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTT401210.16884663619759446TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG400210.1684257926588053Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCT386240.1625466084219209TruSeq Adapter, Index 18 (97% over 34bp)
CAAGCAGAAGACGGCATACGAGATTAGATCGGAAGAGCACACGTCTGAAC385570.16226464325093218RNA PCR Primer, Index 29 (96% over 29bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTGC375660.1580940837815317TruSeq Adapter, Index 18 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCT371040.15614978663232584TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTTG359640.15135217029012954TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTCC349030.14688702034357667TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCCC342110.14397478305515576TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAGC337550.14205573651827724TruSeq Adapter, Index 18 (97% over 38bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCT336350.14155072427173024TruSeq Adapter, Index 11 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCC327890.13799038793357404TruSeq Adapter, Index 18 (97% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGAT326800.1375316684762939TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTC323340.13607554983208342TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCT319930.13464047336481244TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAG312970.13171140233483994TruSeq Adapter, Index 18 (97% over 38bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG306730.12908533865279564Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGTA300760.12657290272622446TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGA296260.12467910680167328TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCAG288800.12153961400230623TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCG280270.11794981861643478TruSeq Adapter, Index 18 (97% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTCG278870.11736063766212997TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCTGT277450.11676303983704937TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGCT276340.11629590350899344TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGCA274440.11549630078529405TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCACG257130.10821149912885387TruSeq Adapter, Index 18 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCCC257110.10820308225807809TruSeq Adapter, Index 18 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG246730.10383472632544673Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCAGG242470.10204193285020494TruSeq Adapter, Index 18 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCGAC238060.1001860128441448TruSeq Adapter, Index 18 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)