FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16249945
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG368677722.68793525147316Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910300.5601865114005001No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTA839110.516377132353371TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACA569350.35037041663833324TruSeq Adapter, Index 9 (97% over 35bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC466700.28720097206482853TruSeq Adapter, Index 9 (100% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACT359750.22138536468892667TruSeq Adapter, Index 9 (97% over 35bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTAC349110.21483765021973922TruSeq Adapter, Index 9 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC331350.2039083824591406Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACC294570.18127445969817127TruSeq Adapter, Index 9 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTC294380.18115753622550723TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTCT282000.1735390489013963TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACAC279170.1717975045454TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG267010.16431440229490007Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACGC266440.1639636318769079TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACG266230.16383440067027918TruSeq Adapter, Index 9 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTA259460.1596682327232492TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTT252770.1555512957120778TruSeq Adapter, Index 9 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT252560.1554220645054491Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACAT242200.14904665831176658TruSeq Adapter, Index 9 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACT234580.1443574116712395TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCT224630.13823431402383207TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACAA223730.13768046599542338TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACGCT222720.13705892543020914TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCC220280.1355573818865233TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTGC210190.12934813010136342TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCTC203160.12502196161279316TruSeq Adapter, Index 9 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACA200990.12368657247762993TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG200420.12333580205963773Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTG199420.12272041536140584TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCA193800.11926194211734256TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACACT183880.11315730607088209TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACGT181020.11139730011393884TruSeq Adapter, Index 9 (97% over 37bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG179760.11062191287416664Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTCA176070.10835113595769094TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACCCT174350.10729267083673207TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACTTT172240.10599420490346274TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCCG169810.10449881522675923Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACC167990.10337881143597717TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTAC166640.1025480393933641TruSeq Adapter, Index 9 (97% over 37bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCG165500.10184649855737972TruSeq Adapter, Index 9 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGAGATCGGAAGAGCACACGTCTGAACTCC164520.10124341959311246Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGTACGCA162620.10007418486647186TruSeq Adapter, Index 9 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)