FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16249945
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG350506821.56972223598295Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2144601.3197583130281365No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810590.4988263037197972No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTTCCATGTGT288910.17779137098617873Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT288030.17724983069173467Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG283060.1741913588015221Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT257300.15833899745506833No Hit
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTTC241490.14860973375602193Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT225280.13863431537768284Illumina Single End PCR Primer 1 (100% over 31bp)
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA177450.10920036960125096Illumina Single End PCR Primer 1 (100% over 26bp)
CGTATGCCGTCTTCAGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG177130.10900344585781675Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTTCCAT175380.10792651913591092Illumina Single End PCR Primer 1 (96% over 33bp)
CTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAG164070.1009664955789081Illumina Single End PCR Primer 1 (100% over 28bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTTCCATGTG162550.10003110779759562Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)