FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24315105
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG334019413.737115262303002Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCG1022780.42063565014422105TruSeq Adapter, Index 2 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710600.29224632178228305No Hit
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAA707570.29100018280817624TruSeq Adapter, Index 2 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGT546040.2245682262116491TruSeq Adapter, Index 2 (97% over 36bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA457760.18826157649740768Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTAC378140.1555164989005805TruSeq Adapter, Index 2 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTAA367500.15114061814662122TruSeq Adapter, Index 2 (97% over 36bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC353770.1454939223992658Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTA348070.1431497005667876TruSeq Adapter, Index 2 (97% over 36bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC333230.13704649846258118Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTAT325500.1338674046441502TruSeq Adapter, Index 2 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTACA310010.12749687899764364TruSeq Adapter, Index 2 (97% over 36bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT296390.1218954226189852Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTTA289200.11893841297415741TruSeq Adapter, Index 2 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTACC279760.11505605260598299TruSeq Adapter, Index 2 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTAAA279570.11497791187823371TruSeq Adapter, Index 2 (97% over 36bp)
GAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG279260.11485041911190594TruSeq Adapter, Index 2 (97% over 35bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAG278200.11441447610446263TruSeq Adapter, Index 2 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTACT267450.10999335598180636TruSeq Adapter, Index 2 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTAAC266620.1096520043816385TruSeq Adapter, Index 2 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTT261000.10734068390821261TruSeq Adapter, Index 2 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTTC250830.1031580986386857TruSeq Adapter, Index 2 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTCC249620.10266046558301929TruSeq Adapter, Index 2 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTGC249130.10245894475882378TruSeq Adapter, Index 2 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTATA247510.10179269223801418TruSeq Adapter, Index 2 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAAGCGTTAA246320.10130328452211085TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)