FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24315105
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG309682812.736231243911963Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1830320.7527501937581598No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470120.193344836470992No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTCAGGTGTGT447170.18390625909285607Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT407840.16773112844875646Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTCA383570.15774967864625714Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG312820.12865253923435657Illumina Single End PCR Primer 1 (100% over 35bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG312730.12861552520542274Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTCAGGT285640.11747430249632893Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG271380.11160963524525187Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT265700.10927363875253675Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA254290.10458108241769878Illumina Single End PCR Primer 1 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)