FastQCFastQC Report
Mon 13 May 2024
EGAF00007947862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80486135
Total Bases12.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG50638886.291627744331368Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3138130.3898969679684582No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTTCACCAA1286670.1598623166586394TruSeq Adapter, Index 12 (97% over 34bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTTCACCAT1135170.1410391988632576TruSeq Adapter, Index 12 (97% over 34bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTTCACCAG838120.10413222103409488TruSeq Adapter, Index 12 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)