FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36836175
Sequences flagged as poor quality0
Sequence length151
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG747248920.285735421769495Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGC2687620.7296142989873405TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGT2297540.6237183963861611TruSeq Adapter, Index 10 (96% over 33bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATC1776400.4822433382401946TruSeq Adapter, Index 9 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTA1585950.4305414446532519TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTT1578790.42859770320887003TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGT1576340.42793259615038753TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGA1538790.4177388124581339TruSeq Adapter, Index 10 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTG1297220.3521592564917503TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCT1242990.33743731535643967TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCA1230500.33404662671952234TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGC1108830.3010165957784705TruSeq Adapter, Index 10 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAA1061560.288184101633788TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGG1042890.28311571437588184TruSeq Adapter, Index 10 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTAT1041130.28263792318284947TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGA1009940.27417070311996294TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTTA962560.26130834702571587TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAT940930.2554364018522553TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGC929720.2523931977193615TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTC928460.25205114266071327TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGTT908690.24668413590716193TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTTT895120.24300025721997465TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAG884300.24006292727190048TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGG878020.23835808142403495TruSeq Adapter, Index 9 (97% over 39bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCG865680.23500811362743282TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCG864990.23482079776198259TruSeq Adapter, Index 9 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGT822320.2232370760536348TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTAA821300.22296017433949103TruSeq Adapter, Index 9 (97% over 39bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCA805250.21860304442575812TruSeq Adapter, Index 9 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCAT805180.2185840413669443TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGTA798610.21680046856113588TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTGT796240.21615707928415479TruSeq Adapter, Index 9 (97% over 39bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC767220.20827895404449567Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCTA740420.20100349724150238TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTTG734430.19937737835157968TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCAA700560.19018261260839378TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGTG698760.18969396252461065TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTAC697820.18943877859196834TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTGA694030.1884098986933361TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG682630.1853151148293763Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCTT675560.18339580588918367TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCTC661470.17957076162223684TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTCT645840.17532765006138665TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCC622750.1690593553755242TruSeq Adapter, Index 9 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGG621090.16860871140936864TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAAT616260.16729750035121724TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCAC608240.16512029275569465TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTGG602320.1635131769245857TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGTC590370.16026908331280323TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGAT577980.1569055419027627TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGCT577450.15676166160031546TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTGC573630.15572463753362015TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGGC571490.15514368687845576TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTAG568370.15429669339989835TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAGT559380.15185615770367036TruSeq Adapter, Index 9 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGC554660.15057480859508351TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGAA547100.14852247824319434TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAAA539000.14632355286617027TruSeq Adapter, Index 9 (97% over 39bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC536280.14558514829512023Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG532360.14452097700154806Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGATG529510.14374728103555812TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCGT516510.14021814154156886TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGCA513960.1395258872562094TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTCA512380.13909696107155534TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGGT503790.13676501428283475TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTTC498470.13532078181298682TruSeq Adapter, Index 9 (97% over 39bp)
GAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA483670.13130299223521444TruSeq Adapter, Index 7 (97% over 35bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAT479300.1301166584206965TruSeq Adapter, Index 9 (97% over 36bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT472770.12834394450563882Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGATT468170.12709517206930415TruSeq Adapter, Index 9 (97% over 39bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT459080.12462748914619935Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGA458400.12444288800343684TruSeq Adapter, Index 9 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAC457000.12406282682716108TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAAC456870.12402753543222118TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGAC435030.1180985810823192TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGAG426930.11589965570529513TruSeq Adapter, Index 9 (97% over 39bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCG426340.11573948706672177TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGATA425010.1153784289492598TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAAG423250.11490063775622741TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGGCC418700.11366543893333117TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCTG413960.11237866037936892TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAGC408570.11091542485070721TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCAG401440.10897982757438848TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCGG394850.1071908253232047TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGAGG387080.1050814857948742TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCGC384550.10439466095489014TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGTCC381910.10367797416534154TruSeq Adapter, Index 9 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCGCCC369960.10043388055355909TruSeq Adapter, Index 9 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)