FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36836175
Sequences flagged as poor quality0
Sequence length151
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG705515119.15277848473681Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20692395.6174100595406555No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGT2679300.7273556497111875Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTG1699430.4613481177130905Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGT1372600.3726228361115127Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT1304660.3541790101713872Illumina Single End PCR Primer 1 (96% over 26bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGTAGATC1291110.3505005609295753Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTA999780.2714125448692759Illumina Single End PCR Primer 1 (96% over 33bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGTAGA993220.2696316867861552Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT984560.2672807369386208No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG907820.2464479550333334No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG700970.19029391623858882Illumina Single End PCR Primer 1 (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT590640.16034238082537072No Hit
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGT579160.1572258791799094Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT484960.13165319146192567Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTAT449640.12206479092902563Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG440340.11954009882947944No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT437230.11869582007360971Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCAGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG414340.11248181984150091Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATGCCGTCTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG413330.11220763285004483Illumina Single End PCR Primer 1 (96% over 33bp)
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGTA383780.10418562730793847Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTATGTGTGTAGATC372640.10116142623385843Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGGTCGTGTAGGGAAAGAGT369790.10038773026786847No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)