FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15822809
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG5194183.2827167413826457Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2629941.6621195389516488No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG335340.21193455599445077No Hit
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA332160.2099247990669672Illumina Single End PCR Primer 1 (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA252260.15942807626635702No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251690.15906783681709108No Hit
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA160480.10142320494420429Illumina Single End PCR Primer 1 (100% over 25bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGTGGCGTGTAGATC160370.1013536850504863Illumina Single End PCR Primer 1 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)