FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17777711
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG343628619.329181355237466Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGA931240.5238244676156565TruSeq Adapter, Index 7 (96% over 33bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA882140.4962056138723371No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGT778530.43792476995491714TruSeq Adapter, Index 7 (96% over 33bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTA479260.26958476262776465TruSeq Adapter, Index 23 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGC444660.2501221895214744TruSeq Adapter, Index 7 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGG418890.23562651007207847TruSeq Adapter, Index 7 (96% over 33bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG341880.19230822235776024Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAC272880.15349557656775947TruSeq Adapter, Index 23 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT268280.15090806684842611Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGCT234990.13218237151003298TruSeq Adapter, Index 23 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGCA222090.12492609425364153TruSeq Adapter, Index 23 (97% over 37bp)
CAAGCAGAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT209810.11801856830724722Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC208060.11703418960967472Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGTT204930.11527355799630223TruSeq Adapter, Index 23 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGTG203580.11451418014388917TruSeq Adapter, Index 23 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCTAGTA198620.11172416966391231TruSeq Adapter, Index 23 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG195240.10982291252231516Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA189980.10686415140846874No Hit
CAAGCAGAAGACGGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT180150.10133475563867586Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)