FastQCFastQC Report
Fri 10 May 2024
EGAF00007947874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12013565
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG3835053.192266408846999Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2723522.2670373032484528No Hit
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT196590.16364001859564584Illumina PCR Primer Index 2 (96% over 31bp)
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC131340.10932641559770143No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA127550.10617164846571354No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)