FastQCFastQC Report
Wed 1 May 2024
EGAF00007947878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21662941
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG422577019.506908134034063Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA917880.4237097816035228No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCT546450.25225106784900536TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAA519350.23974122442562162TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT466070.21514622599027527Illumina PCR Primer Index 2 (96% over 31bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAA446730.2062185369936612TruSeq Adapter, Index 12 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAACT425700.19651071384998003TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAT415210.19166834272410196TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG408890.18875091798477409Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATGC392650.18125424428751388TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATA373530.17242811121537005TruSeq Adapter, Index 1 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCA368300.17001384992000854TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATCT367240.16952453501119724TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATT366230.169058300994311TruSeq Adapter, Index 1 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT363390.16774730633296744Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTT360920.16660711027186936TruSeq Adapter, Index 1 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTA337790.15592988966733556TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAC334080.15421728748649594TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACTT327570.15121215535785285TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAAT326710.15081516401674178TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATAT323360.1492687442577626TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAAC321660.1484839939323105TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAT321620.14846552921877043TruSeq Adapter, Index 12 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTA315700.14573275161484306TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATGT314330.14510033517609636TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACAT312590.1442971201371042TruSeq Adapter, Index 1 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC305120.1408488348834999Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACTA296780.1369989421103995TruSeq Adapter, Index 1 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAA296230.13674505229922382TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATC293170.13533250171341002TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATA292230.13489858094521884TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATT291080.13436772043094242TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAGCT289100.13345371711070994TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAACA288840.13333369647269963TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACAA288570.13320905965630428TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAAA287660.13278898742326817TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAACC283230.13074402039870764TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACTC279810.1291652873910334TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAATG277000.1278681412648449TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATAA276560.12766502941590432TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTC267690.1235704791883983TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG267470.12346892326392803Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACCT266320.12293806274965159TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACA263050.12142857241775251TruSeq Adapter, Index 1 (97% over 37bp)
GGACGTAGGAGGCTGCGATAAGCCTCGGGGAGCTGCCAACCGAGCTTTGA258000.11909740233332124No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATG246010.1135626044496913TruSeq Adapter, Index 1 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTACT241530.11149455653320572TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATAC237870.10980503524429117TruSeq Adapter, Index 1 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAT235170.1085586670803378TruSeq Adapter, Index 1 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAC232070.10712765178098393TruSeq Adapter, Index 12 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAG229060.10573818208709519TruSeq Adapter, Index 12 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATTG228740.1055904643787748TruSeq Adapter, Index 1 (97% over 37bp)
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAA226970.10477340080462759No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTATCA224440.10360550767321942TruSeq Adapter, Index 1 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGCTAAGC220300.10169440982182429TruSeq Adapter, Index 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)