FastQCFastQC Report
Wed 1 May 2024
EGAF00007947879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21662941
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG426538719.6897872731131Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2825571.304333515933963No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA904620.4175887290649963No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTACGCAGTGT640590.29570777116551256Illumina Single End Sequencing Primer (100% over 33bp)
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA622030.2871401440829294Illumina Single End PCR Primer 1 (100% over 26bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTACGCAGTGTAGATC505200.233209332010829Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT472590.21815597429730338Illumina Single End PCR Primer 1 (96% over 26bp)
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA373180.17226654497189464Illumina Single End PCR Primer 1 (100% over 25bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT372740.17206343312295408Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG304290.14046569207754386Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT257750.1189819978736959No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)