FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14752854
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG11678977.916414003690404Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1868261.266371916918584No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAGGAC247400.1676963657337082TruSeq Adapter, Index 13 (97% over 36bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA238030.16134505228615426No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAGGAA228470.15486495019878865TruSeq Adapter, Index 13 (97% over 36bp)
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA204230.13843423109860642No Hit
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC188460.12774477399423867No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAGGAT175330.11884480114830662TruSeq Adapter, Index 13 (97% over 36bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG174950.1185872238686833Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA159890.10837902957624335No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAGGAG159830.10833835947946073TruSeq Adapter, Index 13 (97% over 36bp)
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC150120.10175658215013854No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)