FastQCFastQC Report
Fri 10 May 2024
EGAF00007947882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12448882
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG150037912.052319236378013Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA973590.7820702292784204No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACGAA284780.22875949824249278TruSeq Adapter, Index 3 (96% over 32bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACGAT251550.20206633816594938TruSeq Adapter, Index 3 (96% over 32bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACG245240.1969976099058534TruSeq Adapter, Index 3 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAG200180.16080158844786221TruSeq Adapter, Index 3 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT184680.14835067116870415Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG180020.1446073631350992Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTCAGACGAG176800.14202078548097732TruSeq Adapter, Index 3 (96% over 32bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC141120.11335957718934118Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)