FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12448882
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG142950911.483031166975476Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG869120.6981510468168949No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA729470.5859722985566094No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT230000.18475554672299088Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT176240.14157094588895613Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT159890.12843723637190874Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT151700.12185833233859876No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG132460.1064031292127277Illumina Single End PCR Primer 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)