FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7079149
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG358438550.632992750964846Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1112941.5721381199915414No Hit
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT434470.613731961285177Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC320070.4521306162647516Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGT306310.43269325168886824TruSeq Adapter, Index 9 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCTT252540.3567377943309287TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG251350.3550568013189156Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCTC229430.3240926275177991TruSeq Adapter, Index 6 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGA215230.3040337193072218TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCAT211470.29872234642892814TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCTA210110.29680121155805594TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCCT200470.2831837555615795TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCAC191340.2702867251416802TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTCT179180.25310951923741115TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGACT174910.2470777207825404TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGA169850.23992996898356003TruSeq Adapter, Index 9 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTGC164010.23168038983216765TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCCC162330.2293072232269726TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGC161880.22867155360058108TruSeq Adapter, Index 9 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCCA161580.22824777384965342TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGAGATCGGAAGAGCACACGTCTGAACTCC161440.22805000996588715Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCAA160740.2270611905470559TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG150380.212426663148353Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTACAG149630.2113672137710338Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGCT147820.20881040927377006TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTTT146240.20657850258555088TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCTG136400.1926785267551227TruSeq Adapter, Index 6 (97% over 38bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACA133790.18899164292205178TruSeq Adapter, Index 6 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGG133790.18899164292205178TruSeq Adapter, Index 9 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGACC129870.18345425417659664TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGGCTGAACTCCAG129360.18273382860001958Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTGT129080.1823383008324871TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTAC124040.17521880101690188TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCT117800.16640418219760594TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGCC116580.16468081121050002TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCA114590.1618697388626797TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCGGAACTCCAG110630.1562758461504342Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGACA107980.1525324583505729TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTAT107300.15157189091513684TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCGT105830.14949537013559117TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTTA105760.14939648819370802TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGATC103080.14561072241875403TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACAGATCGGAAGAGCACACGTCTGAACTCCAGT102640.14498917878406006Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA102130.144268753207483No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTCC100260.14162719276003372TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGCA99730.14087851520006148TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATAGGAAGAGCACACGTCTGAACTCCAG99280.14024284557366995Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAAC98890.13969193189746393TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGTC97660.1379544349186604TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTTC94770.13387202331805703TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTGA94710.13378726736787147TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAGC93860.13258655807357636TruSeq Adapter, Index 6 (97% over 38bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG93480.13204977038906796Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTCA89410.12630049176814898TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCAAG89120.12589083800891887Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCAG89010.1257354521002454TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTA85080.12018393736309264TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTAA84050.11872896021824092TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAG84050.11872896021824092TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAA83920.11854532232617226TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGAC83590.11807916460015179TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCC83420.11783902274129278TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAAT82700.11682195133906631TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGGTT82090.11596026584551336TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGATT81990.11581900592853744TruSeq Adapter, Index 6 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGAC81680.11538110018591219TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT80690.11398262700785079Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCCG80130.11319157147278579TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCGA79520.11232988597923281TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGCGC78670.11112917668493769TruSeq Adapter, Index 6 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTTG75690.10691963115905599TruSeq Adapter, Index 6 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAG74930.10584605579003917TruSeq Adapter, Index 6 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCAGAACTCCAG74050.10460296852065128Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGAGTG71510.1010149666294635TruSeq Adapter, Index 6 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)