FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7079149
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG340234148.06144071836883Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1192471.6844821319624719No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG746661.0547312960922282No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT442500.6250751326183416No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT415330.5866948131759906Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT305230.43116764458552853Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG301220.4255031219147951Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGGTCGTGTAGGGAAAGAGT293370.4144142184321873No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTATGGGTGT210120.29681533754975353Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGACAGAG178960.2527987474200642Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTGGGAAAGAGT157960.22313416485512597Illumina Single End PCR Primer 1 (95% over 23bp)
CTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAG153400.21669271264102508Illumina Single End PCR Primer 1 (100% over 28bp)
CGTATGCCGTCTTCAGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG147020.20768032993796287Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT140180.19801815161681158Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCTGTAGGGAAAGAGT134480.18996633634918547No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG122730.17336829610451765Illumina Single End PCR Primer 1 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA122230.17266199651963815No Hit
CGTATGCCGTCATCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG93530.1321204003475559Illumina Single End PCR Primer 1 (100% over 30bp)
GTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT89030.12576370408364057Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGAGGGAAAGAGT87800.12402620710483703No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGTAGGGAAAGAGT87100.12303738768600575No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86620.12235934008452147No Hit
CGTAAGCCGTCATCAGCTAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG81880.11566362001986397Illumina Single End PCR Primer 1 (100% over 30bp)
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76230.10768243471072582No Hit
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTA73880.10436282666179227Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)