FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6136209
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG105669117.220583588336055Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3029874.93769035572289No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA311650.5078868728232693No Hit
GTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276510.45062024451905075No Hit
CTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211530.3447242426064692No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGAA205490.33488103159458876TruSeq Adapter, Index 1 (96% over 32bp)
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197520.3218925561368591No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181640.2960133854632396No Hit
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT168550.27468099603517415Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165990.2705090390500063No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGAT159530.2599813663452467TruSeq Adapter, Index 1 (96% over 32bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA156260.2546523431649737No Hit
TTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154310.25147448530517785No Hit
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC141550.23067988720723168Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGAC131500.21430169669905313TruSeq Adapter, Index 1 (96% over 32bp)
CGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA128810.20991788252323218No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG127240.20735929952842216Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGAG126210.20568073871017103TruSeq Adapter, Index 1 (96% over 32bp)
CTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124380.20269844133405496No Hit
CTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110590.18022528241785768No Hit
CTCCATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82920.13513229422270331No Hit
GTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82090.1337796675439184No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG82050.13371448071602515Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81390.13263889805578655No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGACTC78440.1278313694986595TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA75490.12302384094153246No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGATGC72590.11829779591927198TruSeq Adapter, Index 7 (97% over 36bp)
AGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71740.11691257582654045No Hit
TTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69620.1134576739481983No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA66570.10848717832133814No Hit
GTTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63600.10364705635026447No Hit
TTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63440.10338630903869149No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)